Detection of p56lck Kinase Activity Using Scintillation Proximity Assay in 384-Well Format and Imaging Proximity Assay in 384- and 1536-Well Format

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Assay Assembly for Miniaturized Quantitative PCR in a 384-well Format using the Echo® Liquid Handler

Quantitative PCR (qPCR) has enabled a wide range of real-time applications including comparisons in gene expression, genotyping and SNP analysis. To ensure high data quality, the liquid handling employed in low-volume reactions must be exceptionally precise and accurate. This application note highlights the capabilities of the Echo 555 liquid handler to dispense into 384-well qPCR plates with t...

متن کامل

A cell-based PDE4 assay in 1536-well plate format for high-throughput screening.

The cyclic nucleotide phosphodiesterases (PDEs) are intracellular enzymes that catalyze the hydrolysis of 3,'5'-cyclic nucleotides, such as cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), to their corresponding 5'nucleotide monophosphates. These enzymes play an important role in controlling cellular concentrations of cyclic nucleotides and thus regulate a variet...

متن کامل

ELISPOT Assays in 384-Well Format: Up to 30 Data Points with One Million Cells

Comprehensive immune monitoring requires that frequencies of T cells, producing different cytokines, are measured to establish the magnitude of Th1, Th2, and Th17 components of cell-mediated immunity. Antigen titration provides additional information about the affinity of T cell response. In tumor immunity, it is also advisable to account for determinant spreading by testing multiple epitopes. ...

متن کامل

Scintillation Proximity Assay (SPA): Custom Assay Development and Membrane Validation

G protein-coupled receptors (GPCRs) represent attractive drug targets because they participate in a wide range of physiological processes and are believed to play a role in a variety of disease states. At least a third of all prescription drugs on the market today are molecules acting as agonists or antagonists of the GPCR. Receptor-ligand interaction studies are pivotal in the successful ident...

متن کامل

A luciferase based viability assay for ATP detection in 384-well format for high throughput whole cell screening of Trypanosoma brucei brucei

Background: Human African Trypanosomiasis (HAT) is caused by two trypanosome species, Trypanosoma brucei rhodesiense and Trypanosoma brucei gambiense. Current drugs available for the treatment of HAT have significant issues related to toxicity, administration regimes with limited effectiveness across species and disease stages, thus there is a considerable need to find alternative drugs. A well...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Biomolecular Screening

سال: 2000

ISSN: 1087-0571,1552-454X

DOI: 10.1177/108705710000500403